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KMID : 0379119970250020091
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Korean Journal of Mycology
1997 Volume.25 No. 2 p.91 ~ p.100
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Production of Alkaline Carboxymethyl Cellulase and Xylanase by Batch and Fed - batch Cultures of Alkalophilic Cephalosporium sp. RYM-202
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Abstract
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Production of alkaline carboxymethyl cellulase (CMCase) and xylanase by batch and fed-batch cultures of alkalophilic Cephalosporium sp. RYM-202 was investigated. Of carbon sources tested, wheat bran gave the highest production of those enzymes. The high levels of CMCase on carboxymethyl cellulose and xylanase on birchwood xylan suggest that the biosynthesis of CMCase and xylanase in Cephalosporium sp. RYM-202 is regulated separately at the level of enzyme induction. The temperature and pH for maximal production of those enzymes was 20℃ and 9.0, respectively. High concentration of wheat bran in batch fermentation resulted in the lower and delayed production of the enzymes by catabolite repression. In fed-batch fermentation with controlled feeding of 5 % final wheat bran concentration, the highest activities of CMCase and xylanase were 0.39 and 9.2 units/㎖, respectively, and 1.22 and 1.36 times higher respectively than those in batch fermentation on 5% wheat bran.
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